Aula Internacional 1 Audio Cd
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Aulas de Salsa & Sautera.
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Aula de Salsa Salsa.
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Category:Rock ballads1. Field of the Invention
This invention relates to the identification of molecular changes at specific loci in a population of cells. The invention particularly relates to the detection of cancerous, pre-cancerous or hyperproliferative cells in a human.
2. Related Background Art
Detection of early-stage cancer, precancer, and hyperproliferative cells have long been sought. Most modalities of detection have used parameters related to the character of the cell: mass, shape, size, internal structure, molecular content, metabolic activity, etc. Few methods have been developed to detect the presence of cancerous or hyperproliferative cells, even those based on detection of peculiar physical properties of cells.
One method that has been developed to detect cancerous cells is ELISA. In this technique, a labelled polyclonal antibody specific for a cancer-associated antigen is applied to a sample of cells obtained from a patient’s body. The sample is then processed to remove the antigens of normal cells and to retain the polyclonal antibody in the sample. Presence of cancer cells is detected by applying a second labelled antibody specific for an epitope on the same cancer-associated antigen. The labelled polyclonal antibody is a “capture antibody” which binds to an immobilised antigen, and thus quantitates the presence of the cancer-associated antigen. The labelled antibody specific for the cancer-associated antigen is a “detect antibody” and quantitates the concentration of the cancer-associated antigen in the sample.
The most common cancer biomarker detected with ELISA techniques is the cellular adhesion molecule EpCAM, or CD326, which is expressed by epithelial cells, but not by embryonic, mesenchymal, or hematopoietic cells. EpCAM-based ELISA assays are used to detect and to quantify circulating tumour cells in blood and bone marrow, for diagnostic and prognostic purposes.
Accordingly, the present invention provides a method of detecting, diagnosing and prognosticating the presence of cancer or hyperproliferative cells, in which a first sample of material is obtained from a patient’s body, and the sample is treated so as to remove the normal material and leave the cancer-associated or hyperproliferative material intact. The sample is then processed to